EXPRESSION OF NF-ΚBMRNA IN ASTHMA RATS LUNG BASED ON THE EFFECT OF DIFFERENT TCM TREATMENT
Journal: Asian Pacific Journal of Microbiology Research (AJMR)
Author: Li Xueliang, Xu Zhaoxia, Qian Peng, Li Na, Cheng Lijun, Yan Haixia, Xu Jin, Guo Rui, Wang Yiqin
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
To detect expression of NF-κB gene in the asthmatic rats lung tissue to explore the effect of NF-κB in asthma rats airway remodeling based on Real-time quantitative PCR. Then to research the effect of different TCM interventions for its role. Asthma rat models were prepared by injecting ovalbumin (OVA) into the abdominal cavity and inhalation of aerosol, and intervented by the different TCM treatment methods，example for body resistance-strengthening, lung-diffusing, lung-diffusing and body resistance-strengening. Then detected expression of NF-κB gene in the asthmatic rats lung tissue based on Real-time quantitative PCR. And compared lung coefficient between the rats in each group. The results showed that the three prescriptions can all reduce the expression of NF-ΚB in the lung tissue, there is statistical significant difference between model group and lung-diffusing and lung-diffusting and body resistance-strengthening group. The three treatment methods can reduce the lung coefficient. NF-κBmRNA is Gene NF-κB in airway remodeling in asthmatic rats has important value and different TCM treatment is effect to it. This is consistent with TCM theory understanding to asthma. The pathogenesis of asthma is deficiency of healthy Qi and exuberance of evils, then the treatment principles should strengthen healthy Qi to remove toxic substance.