Matrix Science Medica (MSM)

A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot-and-mouth dis- ease in District Faisalabad, Pakistan during the Year 2016

December 21, 2017 Posted by mieyo In Matrix Science Medica (MSM)

ABSTRACT

 

A Reverse transcription-polymerase chain reaction (RT-PCR) based detection of foot and mouth disease in District Faisalabad, Pakistan during the Year 2016

Matrix Science Medica (MSM)
Author: Waqas Ali, Mudasser Habib, Sanaullah Sajid, Rai Shafqat Ali Khan, M. Usman Mazhar, Irfan Ullah Khan, Uneeb Saliha, Muhammad Farooq, M. Salah Ud Din Shah, Hafiz Muhammad Muzammil

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/msm.01.2017.27.29

Foot and mouth disease is an economically devastating disease of livestock that mainly effect cloven-hoofed animals i.e. sheep, goat, cattle, pig, buffalo, deer etc. The aim of this study was to determine the serotypes circulating in the region during 2016. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the confirmation of FMDV genome. Out of total 72 samples, 65 were found positive which were then serotyped into type O (n=30), Asia1 (n=19) and A (n=5). Some samples (n=5) were found positive for more than one serotype that were subjected to reverse transcriptase loop-mediated isothermal amplification assay (RT-LAMP) for serotype determination.
Pages 27-29
Year 2017
Issue 1
Volume 1

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